631-02-013 / 631-02-013 ISMRM Abstract

Hybrid ¹H-MRS and CEST-MRI approach for in vivo quantification of neurogenesis-linked bioimaging marker in embryo brain

Primary: Preclinical Animal & in vitro MR - Biomarkers

Secondary: Contrast Mechanisms - Hybrid Imaging

631-02-013 · Everything About Contrast Mechanism · Thursday, 14 May, 1:40 PM–3:16 PM · Roof Terrace

Keywords: MRS Biomarker Animal mouse model CEST MRI in vivo Neurogenesis

Accepted

Mariano G Uberti ¹, Aditya N Bade², Yutong Liu^1,2

¹Department of Radiology, University of Nebraska Medical Center, Omaha, United States of America

²Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, United States of America

Presenting Author: Mariano G Uberti

Synopsis

Motivation:

Goals:

Approach:

Results:

Full abstract & presentation

The full text, figures, and any recorded presentation for this abstract are not shown here. Log in if you are a member or registered attendee with access.

Full abstracts, figures, and presentations for Cape Town - 2026 ISMRM-ISMRT Annual Meeting and Exhibition are available to registered attendees. This content becomes freely available to the public roughly two years after the meeting.

To request or purchase access, contact the ISMRM Central Office at info@ismrm.org.

References

1. Chakrabarti, L., Galdzicki, Z. & Haydar, T. F. Defects in embryonic neurogenesis and initial synapse formation in the forebrain of the Ts65Dn mouse model of Down syndrome. J Neurosci 27, 11483-11495 (2007). https://doi.org:10.1523/JNEUROSCI.3406-07.2007

2. Stoner, R. et al. Patches of disorganization in the neocortex of children with autism. N Engl J Med 370, 1209-1219 (2014). https://doi.org:10.1056/NEJMoa1307491

3. Hong, S. et al. Defective neurogenesis and schizophrenia-like behavior in PARP-1-deficient mice. Cell Death Dis 10, 943 (2019). https://doi.org:10.1038/s41419-019-2174-0

4. Ross, E. J., Graham, D. L., Money, K. M. & Stanwood, G. D. Developmental consequences of fetal exposure to drugs: what we know and what we still must learn. Neuropsychopharmacology 40, 61-87 (2015). https://doi.org:10.1038/npp.2014.147

5. Kandel, P. et al. Oleic acid is an endogenous ligand of TLX/NR2E1 that triggers hippocampal neurogenesis. Proc Natl Acad Sci U S A 119, e2023784119 (2022). https://doi.org:10.1073/pnas.2023784119

6. Garcia-Cerro, S. et al. Prenatal Administration of Oleic Acid or Linolenic Acid Reduces Neuromorphological and Cognitive Alterations in Ts65dn Down Syndrome Mice. J Nutr 150, 1631-1643 (2020). https://doi.org:10.1093/jn/nxaa074

7. Velasco, A., Tabernero, A. & Medina, J. M. Role of oleic acid as a neurotrophic factor is supported in vivo by the expression of GAP-43 subsequent to the activation of SREBP-1 and the up-regulation of stearoyl-CoA desaturase during postnatal development of the brain. Brain Res 977, 103-111 (2003). https://doi.org:10.1016/s0006-8993(03)02772-0

8. Shi, Y., Evans, R. M. & Gage, F. H. Oleic acid regulates hippocampal neurogenesis as a TLX ligand. Proc Natl Acad Sci U S A 119, e2203038119 (2022). https://doi.org:10.1073/pnas.2203038119

9. Manganas, L. N. et al. Magnetic resonance spectroscopy identifies neural progenitor cells in the live human brain. Science 318, 980-985 (2007). https://doi.org:10.1126/science.1147851

10. Tabernero, A., Lavado, E. M., Granda, B., Velasco, A. & Medina, J. M. Neuronal differentiation is triggered by oleic acid synthesized and released by astrocytes. J Neurochem 79, 606-616 (2001). https://doi.org:10.1046/j.1471-4159.2001.00598.x

11. Vidal, V. et al. Early postnatal oleic acid administration enhances synaptic development and cognitive abilities in the Ts65Dn mouse model of Down syndrome. Nutr Neurosci 25, 1400-1412 (2022). https://doi.org:10.1080/1028415X.2020.1861897

12. Uberti M; Bade AN; Liu Y. Towards in vivo detection of bioimaging marker of neurogenesis process in murine embryo brain. International Society for Magnetic Resonance in Medicine. 2025 Annual Meeeting. Honolulu, Hawaii (USA).

13. Lanz, B. et al. Magnetic resonance spectroscopy in the rodent brain: Experts' consensus recommendations. NMR Biomed, e4325 (2020). https://doi.org:10.1002/nbm.4325

14. Tkác I, Starcuk Z, Choi IY, Gruetter R. In vivo 1H NMR spectroscopy of rat brain at 1 ms echo time. Magn Reson Med. 1999 Apr;41(4):649-56. doi:10.1002/(sici)1522-2594(199904)41:4<649::aid-mrm2>3.0.co;2-g. PMID: 10332839. [doi] [pmid]

15. Gruetter R, Tkác I. Field mapping without reference scan using asymmetric echo-planar techniques. Magn Reson Med. 2000 Feb;43(2):319-23. doi:10.1002/(sici)1522-2594(200002)43:2<319::aid-mrm22>3.0.co;2-1. PMID: 10680699. [doi] [pmid]

16. MRspa: Magnetic Resonance signal processing and analysis: https://www.cmrr.umn.edu/downloads/mrspa/

17. Provencher SW. Estimation of metabolite concentrations from localized in vivo proton NMR spectra. Magn Reson Med. 1993 Dec;30(6):672-9. PubMed [citation] PMID:8139448 [pmid]

Cite this abstract

http://echo.ismrm.org/p/ISMRM2026/631-02-013