Cape Town - 2026 ISMRM-ISMRT Annual Meeting and Exhibition
9 May 2026 – 14 May 2026 · Cape Town, South Africa
460-06-012 Registered Abstract

Validation and comparison of experimental measures of glymphatic clearance in the rat brain

Accepted
Ben N Leverton 1, Yolanda Ohene2,3, William Morrey2,4, Ross A Little1, Juan Sebastian Bermudez2,4, Ben Dickie2,4,5
1Preclinical Imaging, Faculty of Biology Medicine and Health, The University of Manchester, Manchester, UK., University of Manchester, Manchester, United Kingdom
2Geoffrey Jefferson Brain Research Centre, Manchester Academic Health Science Centre, Northern Care Alliance NHS Foundation Trust, The University of Manchester, Manchester, UK., University of Manchester, Manchester, United Kingdom
3Division of Psychology, Communication and Human Neuroscience, Faculty of Biology, Medicine and Health, The University of Manchester, University of Manchester, Manchester, United Kingdom
4Division of Informatics Imaging & Data Sciences, University of Manchester, Manchester, United Kingdom
5Division of Pharmacy and Optometry, Faculty of Biology, Medicine, and Health, The University of Manchester, University of Manchester, Manchester, United Kingdom
Presenting Author: Ben N Leverton

Synopsis

Motivation:
Goals:
Approach:
Hypothesis:
Full abstract & presentation

The full text, figures, and any recorded presentation for this abstract are not shown here. Log in if you are a member or registered attendee with access.

Full abstracts, figures, and presentations for Cape Town - 2026 ISMRM-ISMRT Annual Meeting and Exhibition are available to registered attendees. This content becomes freely available to the public roughly two years after the meeting.

To request or purchase access, contact the ISMRM Central Office at info@ismrm.org.

Log in

References

1. Humberto Mestre, Lauren M Hablitz, Anna LR Xavier, Weixi Feng, Wenyan Zou, Tinglin Pu, Hiromu Monai, Giridhar Murlidharan, Ruth M Castellanos Rivera, Matthew J Simon, Martin M Pike, Virginia Plá, Ting Du, Benjamin T Kress, Xiaowen Wang, Benjamin A Plog, Alexander S Thrane, Iben Lundgaard, Yoichiro Abe, Masato Yasui, John H Thomas, Ming Xiao, Hajime Hirase, Aravind Asokan, Jeffrey J Iliff, Maiken Nedergaard (2018) Aquaporin-4-dependent glymphatic solute transport in the rodent brain eLife 7:e40070 https://doi.org/10.7554/eLife.40070 [doi]
2. Hauglund NL, Andersen M, Tokarska K, Radovanovic T, Kjaerby C, Sørensen FL, Bojarowska Z, Untiet V, Ballestero SB, Kolmos MG, Weikop P, Hirase H, Nedergaard M. Norepinephrine-mediated slow vasomotion drives glymphatic clearance during sleep. Cell. 2025 Feb 6;188(3):606-622.e17. doi: 10.1016/j.cell.2024.11.027. Epub 2025 Jan 8. PMID: 39788123; PMCID: PMC12340670. [doi] [pmid]
3. Miao, A., Luo, T., Hsieh, B. et al. Brain clearance is reduced during sleep and anesthesia. Nat Neurosci 27, 1046–1050 (2024). https://doi.org/10.1038/s41593-024-01638-y [doi]
4. Tirado-García, P., Ferreiro, A., González-Alday, R. et al. Aquaporin-4 inhibition alters cerebral glucose dynamics predominantly in obese animals: an MRI study. Sci Rep 15, 15649 (2025). https://doi.org/10.1038/s41598-025-99641-1 [doi]
5. Wang Z, Tan S, Lu K, et al. The contributions of aquaporin-4 to water exchange across the blood–brain barrier measured by filter-exchange imaging. Magn Reson Med. 2025; 1-14. doi: 10.1002/mrm.70049 [doi]
6. Benveniste H, Lee H, Ding F, Sun Q, Al-Bizri E, Makaryus R, Probst S, Nedergaard M, Stein EA, Lu H. Anesthesia with Dexmedetomidine and Low-dose Isoflurane Increases Solute Transport via the Glymphatic Pathway in Rat Brain When Compared with High-dose Isoflurane. Anesthesiology. 2017 Dec;127(6):976-988. doi: 10.1097/ALN.0000000000001888. PMID: 28938276; PMCID: PMC5685871. [doi] [pmid]
7. Igarashi H, Huber VJ, Tsujita M, Nakada T. Pretreatment with a novel aquaporin 4 inhibitor, TGN-020, significantly reduces ischemic cerebral edema. Neurol Sci. 2011 Feb;32(1):113-6. doi: 10.1007/s10072-010-0431-1. Epub 2010 Oct 6. PMID: 20924629; PMCID: PMC3026762. [doi] [pmid]
8. Ohene, Y., Harris, W.J., Powell, E. et al. Filter exchange imaging with crusher gradient modelling detects increased blood–brain barrier water permeability in response to mild lung infection. Fluids Barriers CNS 20, 25 (2023). https://doi.org/10.1186/s12987-023-00422-7 [doi]
9. Berks et al., (2021). Madym: A C++ toolkit for quantitative DCE-MRI analysis. Journal of Open Source Software, 6(66), 3523, https://doi.org/10.21105/joss.03523 [doi]
10. Fukuda M, Vazquez AL, Zong X, Kim SG. Effects of the α₂-adrenergic receptor agonist dexmedetomidine on neural, vascular and BOLD fMRI responses in the somatosensory cortex. Eur J Neurosci. 2013 Jan;37(1):80-95. doi: 10.1111/ejn.12024. Epub 2012 Oct 29. PMID: 23106361; PMCID: PMC3538949. [doi] [pmid]
11. Benveniste, Helene M.D., Ph.D.; Lee, Hedok Ph.D.; Ding, Fengfei M.D., Ph.D.; Sun, Qian Ph.D.; Al-Bizri, Ehab M.D.; Makaryus, Rany M.D.; Probst, Stephen M.D.; Nedergaard, Maiken M.D., Ph.D.; Stein, Elliot A. Ph.D.; Lu, Hanbing Ph.D.. Anesthesia with Dexmedetomidine and Low-dose Isoflurane Increases Solute Transport via the Glymphatic Pathway in Rat Brain When Compared with High-dose Isoflurane. Anesthesiology 127(6):p 976-988, December 2017. | DOI: 10.1097/ALN.0000000000001888 [doi]

Cite this abstract