665-04-002 ISMRM Abstract

Feasibility of Dynamic 13C-NMR measurements in Patient-Derived Midbrain Organoids to Probe Metabolism in Parkinson’s Disease

Primary: Preclinical Animal & in vitro MR - Biomarkers

Secondary: Preclinical Animal & in vitro MR - Biology, Models, Methods

665-04-002 · MR in Organoid/Phantom/Plant/Serum · Thursday, 14 May, 2:35 PM–3:30 PM · Digital Posters Row F

Keywords: Parkinson's Disease NMR spectroscopy Mitochondrial dysfunction Hyperpolarized 13C NMR

Accepted

Marta M Pokotylo ^1,2, Josh Philipp Peters³, Farhad Haj Mohamad³, Franziska Rudolph⁴, Philip Seibler⁴, Jan-Bernd Hövener³, Andrey Pravdivtsev³, Jannik Prasuhn^2,4,5,6

¹Department of Neurology, University of Luebeck, Luebeck, Germany

²Section of Movement Disorders, Department of Neurology, University Hospital Schleswig-Holstein, Kiel, Germany

³Section Biomedical Imaging, Dept. Radiology, Kiel University, Kiel, Germany

⁴Institute of Neurogenetics, University of Luebeck, Luebeck, Germany

⁵Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, United States of America

⁶F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, United States of America

Presenting Author: Marta M Pokotylo

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References

1. Pokotylo M, Brüggemann N, Prasuhn J. Metabolic Dysregulation in Parkinson’s Disease: Non-Oxidative Phosphorylation and Its Role in Brain Energy Metabolism. Aging Dis. 2025;0. doi: 10.14336/AD.2025.0619 [doi]

2. Ferrari A, Peters J, Anikeeva M, Pravdivtsev A, Ellermann F, Them K, et al. Performance and reproducibility of 13C and 15N hyperpolarization using a cryogen-free DNP polarizer. Sci Rep. 2022 July 8;12(1):11694. doi: 10.1038/s41598-022-15380-7 [doi]

3. Peters JP, Assaf C, Mohamad FH, Beitz E, Tiwari S, Aden K, et al. Yeast Solutions and Hyperpolarization Enable Real-Time Observation of Metabolized Substrates Even at Natural Abundance. Anal Chem. 2024 Oct 29;96(43):17135–44. doi: 10.1021/acs.analchem.4c02419 [doi]

4. Lian B, Zhang J, Yin X, Wang J, Li L, Ju Q, et al. SIRT1 improves lactate homeostasis in the brain to alleviate parkinsonism via deacetylation and inhibition of PKM2. Cell Rep Med. 2024 Aug;5(8):101684. doi: 10.1016/j.xcrm.2024.101684 [doi]

5. Mallet D, Dufourd T, Decourt M, Carcenac C, Bossù P, Verlin L, et al. A metabolic biomarker predicts Parkinson’s disease at the early stages in patients and animal models. J Clin Invest. 2022 Feb 15;132(4):e146400. doi: 10.1172/JCI146400 [doi]

Cite this abstract

http://echo.ismrm.org/p/ISMRM2026/665-04-002