Cape Town - 2026 ISMRM-ISMRT Annual Meeting and Exhibition
9 May 2026 – 14 May 2026 · Cape Town, South Africa
301-03-003 ISMRM Abstract

State-Dependent Functional Connectivity Revealed by Multimodal Awake fMRI–Calcium Imaging in Mice

Primary: Preclinical Animal & in vitro MR - Small Animals
Secondary: Brain Function and fMRI - Functional Connectivity
301-03-003 · Neuroimaging and Spectroscopy in Experimental Models · Monday, 11 May, 1:50 PM–3:40 PM · Hall 1A
Keywords: fMRI (resting state) Awake animal fMRI Multimodal neuroimaging Calcium wide-field imaging Awake fMRI
Accepted
Francesca Mandino 1, Taekyung Kang2, Xilin Shen1, Corey Horien3, Xenophon Papademetris1,2,4,5, Stephen M Strittmatter6,7,8, Evelyn Lake1,2,4
1Yale School of Medicine, Department of Radiology & Biomedical Imaging, New Haven, United States of America
2Biomedical Engineering, Yale School of Medicine, New Haven, United States of America
3Department of Psychiatry, University of Pennsylvania, Philadelphia, United States of America
4Biomedical Imaging Institute, Yale University, New Haven, United States of America
5Biomedical Informatics & Data Science, Yale School of Medicine, New Haven, United States of America
6Cellular Neuroscience, Neurodegeneration and Repair, Yale School of Medicine, New Haven, United States of America
7Neurology, Yale School of Medicine, New Haven, United States of America
8Neuroscience, Yale School of Medicine, New Haven, United States of America
Presenting Author: Francesca Mandino

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References

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2. Mandino, F., Vujic, S., Grandjean, J. & Lake, E. M. R. Where do we stand on fMRI in awake mice? Cereb Cortex 34 (2024). https://doi.org/10.1093/cercor/bhad478 [doi]
3. Madularu, D. et al. A non-invasive restraining system for awake mouse imaging. J Neurosci Methods 287, 53-57 (2017). https://doi.org/10.1016/j.jneumeth.2017.06.008 [doi]
4. Gozzi, A. et al. Charting the path in rodent functional neuroimaging. Imaging Neurosci (Camb) 3 (2025). https://doi.org/10.1162/IMAG.a.12 [doi]
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6. Lake, E. M. R. et al. Simultaneous cortex-wide fluorescence Ca(2+) imaging and whole-brain fMRI. Nat Methods 17, 1262-1271 (2020). https://doi.org/10.1038/s41592-020-00984-6 [doi]
7. Barson, D. et al. Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits. Nat Methods 17, 107-113 (2020). https://doi.org/10.1038/s41592-019-0625-2 [doi]
8. Hamodi, A. S., Martinez Sabino, A., Fitzgerald, N. D., Moschou, D. & Crair, M. C. Transverse sinus injections drive robust whole-brain expression of transgenes. Elife 9 (2020). https://doi.org/10.7554/eLife.53639 [doi]
9. Mandino, F. et al. Aging-dependent loss of functional connectivity in a mouse model of Alzheimer's disease and reversal by mGluR5 modulator. Mol Psychiatry 30, 1730-1745 (2025). https://doi.org/10.1038/s41380-024-02779-z [doi]
10. Mandino, F. et al. Multimodal identification of the mouse brain using simultaneous Ca(2+) imaging and fMRI. Commun Biol 8, 665 (2025). https://doi.org/10.1038/s42003-025-08037-4 [doi]
11. Desrosiers-Grégoire, G., Devenyi, G. A., Grandjean, J. & Chakravarty, M. M. A standardized image processing and data quality platform for rodent fMRI. Nature Communications 15, 6708 (2024).
12. Vafaii, H. et al. Multimodal measures of spontaneous brain activity reveal both common and divergent patterns of cortical functional organization. Nat Commun 15, 229 (2024). https://doi.org/10.1038/s41467-023-44363-z [doi]
13. Grandjean, J. et al. A consensus protocol for functional connectivity analysis in the rat brain. Nat Neurosci 26, 673-681 (2023). https://doi.org/10.1038/s41593-023-01286-8 [doi]
14. Xu, N. et al. QPPLab: A generally applicable software package for detecting, analyzing, and visualizing large-scale quasiperiodic spatiotemporal patterns (QPPs) of brain activity. bioRxiv (2023). https://doi.org/10.1101/2023.09.25.559086 [doi]

Cite this abstract

http://echo.ismrm.org/p/ISMRM2026/301-03-003